Journal: Nanoscale

Article Title: Tissue Factor-Specific Ultra-bright SERRS Nanostars for Raman Detection of Pulmonary Micrometastases

doi: 10.1039/c6nr08217c

Figure Lengend Snippet: (a) H&E, anti–mouse-CD31 and anti–human-TF (CD142) staining of healthy and metastatic lungs (Scale bars = 2 mm). (b) Direct comparison of BLI, Raman map of αTF-SERRS-NPs, and 3D H&E in a mouse with breast cancer lung metastases. Note the high degree of correlation between the Raman map, BLI, and 3D histology.

Article Snippet: Tissue sections of 5 μm thickness were stained for immunohistochemistry on a Leica Bond RX (Leica Biosystems) with 0.5 μg/ml CD31 rat anti-mouse monoclonal Ab (Dianova cat#DIA-310) or 1 μg/ml CD142 rabbit anti-human TF polyclonal Ab (Thermofisher cat#PA5-27278) for 1 hour on Protocol F. The sections were pre-treated with Leica Bond ER2 Buffer (Leica Biosystems) for 20 min at 100 °C before each staining.

Techniques: Staining, Comparison

Journal: Nanoscale

Article Title: Tissue Factor-Specific Ultra-bright SERRS Nanostars for Raman Detection of Pulmonary Micrometastases

doi: 10.1039/c6nr08217c

Figure Lengend Snippet: (a) H&E, anti–mouse-CD31 and anti–human-TF (CD142) staining of healthy and metastatic lungs (Scale bars = 2 mm). (b) Direct comparison of BLI, Raman map of αTF-SERRS-NPs, and 3D H&E in a mouse with breast cancer lung metastases. Note the high degree of correlation between the Raman map, BLI, and 3D histology.

Article Snippet: Tissue sections of 5 μm thickness were stained for immunohistochemistry on a Leica Bond RX (Leica Biosystems) with 0.5 μg/ml CD31 rat anti-mouse monoclonal Ab (Dianova cat#DIA-310) or 1 μg/ml CD142 rabbit anti-human TF polyclonal Ab (Thermofisher cat#PA5-27278) for 1 hour on Protocol F. The sections were pre-treated with Leica Bond ER2 Buffer (Leica Biosystems) for 20 min at 100 °C before each staining.

Techniques: Staining